Table 1 Bacterial strains and plasmids

CC118 λpir

Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE(Am) recA1 λpir phage lysogen

[64]

P. putida

PaW85

Wild-type, isogenic to KT2440

[26]

PaWcolR

PaW85 colR::Km^r

[22]

PaWoprB1

PaW85 oprB1::Sm^r

[23]

PaWcolR-oprB1

PaWcolR oprB1::Sm^r

[23]

PaWoprB1-tacB1

PaWoprB1 + oprB1 under the control of tac promoter and lacI^q repressor (Sm^r Gm^r)

This study

PaWcolR-oprB1-tacB1

PaWcolR-oprB1 + oprB1 under the control of tac promoter and lacI^q repressor (Sm^r Gm^r)

This study

PaWcrc

PaW85 crc::Tet^r

This study

PaWoprB1-tacB1-crc

PaWoprB1-tacB1 crc::Tet^r (Sm^r Gm^r Tet^r)

This study

Plasmids

mTn5SSgusA40

Delivery plasmid for mini Tn5 Sm (Ap^r Sm^r)

[65]

pRK2013

Helper plasmid for conjugal transfer of mTn5SSgusA40 (Km^r)

[66]

pKTlacZS/C

Promoter probe plasmid pKTlacZ containing tnpA promoter of Tn4652 fused with lacZ

[35]

p9TT_BlacZ

Promoter probe plasmid (Cm^r Ap^r)

[23]

p9TT1015

p9TT_BlacZ containing gtsA promoter fused with lacZ (Cm^r Ap^r)

This study

pBRlacItac

Expression vector containing P_tac promoter and lacI^q repressor in pBR322 (Ap^r)

[67]

pBRlacItac/oprB1

pBRlacItac containing oprB1 as a HindIII-XbaI fragment under the P_tac promoter (Ap^r)

This study

pUCNotKm

pUC18Not derivative with Km^r gene instead of Ap^r (Km^r)

R. Teras

pUCNotKm/tacoprB1

pUC18NotKm containing BamHI fragment with lacI^q-P_tac-oprB1 cassette (Km^r)

This study

pBK-miniTn7-ΩGm

pUC19-based delivery plasmid for miniTn7-ΩGm (Ap^r Gm^r)

[68]

pminiTn7Gm/tacoprB1

pBK-miniTn7-ΩGm containing NotI fragment with lacI^q-P_tac-oprB1 cassette (Ap^r Gm^r)

This study

pCRC10

pKNG101 containing sucB and crc interrupted with tetracycline resistance gene (Sm^r Tet^r)

[32]