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Figure 5 | BMC Microbiology

Figure 5

From: The ColRS system is essential for the hunger response of glucose-growing Pseudomonas putida

Figure 5

Comparison of lysis of peripheral and central subpopulations of P. putida PaW85 wild-type (wt) and colR -deficient (colR) strains grown on solid glucose medium. A. Representation of a Petri plate with three growth sectors of bacteria and subpopulations sampled for β-galactosidase analysis. Unmasked β-galactosidase activity was assayed from the cells of peripheral subpopulation (area encircled by the dotted line and indicated by the white arrow) and from central one (indicated by the black arrow). Black circles indicate the areas sampled for the measurement of residual glucose concentration in the medium (data is presented in Table 3). The degree of lysis is presented as unmasked β-galactosidase activity which was measured from bacteria grown either 24, 48 or 72 hours on solid media with 0.2% (B), 0.4% (C) or 0.8% (D) of glucose (glc) as the carbon source.

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