Figure 3

Cell growth (A), total DNA (B), total RNA (C) and total protein (D) (mean ± SE) per ml in B. burgdorferi B31 cultured in BSK-H at 34°C in the presence (solid circle) or absence (open circle) of 6% rabbit serum, and in BSK-H at 23°C in the presence of 6% of rabbit serum (triangle); Data point symbols obscure the error bars in some cases; Mean (± SE) DNA (E), RNA (F) and protein (G) per B. burgdorferi B31 cell after culture in BSK-H containing 6% of rabbit serum at 34°C (black bar), in BSK-H containing no serum at 34°C (white bar), or in BSK-H containing 6% of rabbit serum at 23°C (gray bar). Data from pooled values from washed exponentially growing cells (days 3-5 for the 34°C condition and day 11 for the 23°C condition) or washed stationary cells (days 7-11 for the 34°C condition and days 14-26 for the 23°C condition), and represent means of 4-8 values for DNA, RNA and protein from cells in exponential phase, and 12-18 values for cells in stationary phase. Data were analyzed by one-way analysis of variance with a Tukey-Kramer multiple comparisons post-test. *, DNA per cell was significantly greater (P < 0.05) in exponentially growing B. burgdorferi B31 cultured at 34°C in the presence of 6% rabbit serum than in stationary phase B. burgdorferi under any culture condition examined. **, RNA per cell was significantly lower (P < 0.05) in stationary phase B. burgdorferi B31 cultured at 23°C in the presence of 6% rabbit serum than in B. burgdorferi cultured at 34°C in the presence or absence of 6% rabbit serum.