Quantitative Reverse Transcription PCR analysis of (A) cpcA, trpC and aroC , (B) sirZ and sirP in wild type (wt) and a cpcA -silenced (cpcA-sil) isolate of Leptosphaeria maculans. Six replicates of each isolate were grown in Tinline for eight days and then mycelia were washed and then transferred to fresh Tinline media for 5 h with 5 mM 3AT (+) or without 3AT (-). RNA was isolated from all treatments, cDNA prepared and q RT-PCR carried out. Transcript level is normalised to that of actin. Values are means ± SE of triplicate reactions of three independent biological samples. Asterisks mark values that have a significant increase (p < 0.05) in mean transcription levels compared to controls without 3AT.