A) The cpcA locus of Leptosphaeria maculans. The conserved leucine zipper region at the C-terminus of cpcA is dark grey. The open boxes indicate the upstream Open Reading Frames uORF1 and uORF2 in the 5' leader region. The black dot preceding them represents the putative cross-pathway control element (CPRE) whose sequence is 5'TGACTCA3'. B) Alignment of the deduced amino acid sequence of uORF2 with counterparts in the leader sequences of Aspergillus fumigatus (Af) cpcA (GenBank XP_751584.1) and A. nidulans (An) cpcA (GenBank AF302935). Black boxes with white text denote amino acids identical in two of the three fungal species. Grey boxes with white text mark conserved changes. Gaps are introduced to optimize alignment. C) Alignment of the deduced amino acid sequence of the C-terminus conserved leucine zipper region with that of A. fumigatus CpcA and A. nidulans CpcA. The thick black line denotes the bZIP transcription factors basic domain signature (PS00036). Asterisks mark positions where conserved leucine residues characteristic of a leucine zipper (L-x(6)-L-x(6)-L-x(6)-L) should be found.