Quantitative Reverse Transcription PCR analysis of the sirodesmin pathway-specific transcription factor, sirZ . in Leptosphaeria maculans wild type (IBCN 18) and sirodesmin-deficient mutants GTA6, GTA7 and GTA9. Cultures were grown for six days in 10% V8 juice. Gene expression level is normalised to that of actin. Values are means ± SE of triplicate reactions of three independent biological samples. This experiment was repeated twice and consistent results were seen.