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Table 2 Comparison of back-staining and pre-staining of Anodisc membranes in VLP enumeration of three sample types

From: A protocol for enumeration of aquatic viruses by epifluorescence microscopy using Anodisc™ 13 membranes

Sample Filtera Staining method Rinse VLP b CV c
  Ano 25 Back No 1.32 × 106 (0.08) 5.7
  Ano 25 Back Yes 1.32 × 106 (0.10) 7.5
Cyanophage lysate Ano 25 Pre No 1.63 × 106 (0.07) 4.5
  Ano 25 Pre Yes 1.54 × 106 (0.15) 9.6
  Ano 13 Pre No 1.29 × 106 (0.13) 10.1
  Ano 13 Pre Yes 1.26 × 106 (0.07) 5.8
  Ano 25 Back No 9.59 × 105 (1.86) 19.4
  Ano 25 Back Yes 1.66 × 105 (0.37) 22.5
Sargasso Sea water Ano 25 Pre No 7.50 × 105 (1.30) 17.3
  Ano 25 Pre Yes 1.75 × 105 (0.17) 9.7
  Ano 13 Pre No 5.93 × 105 (1.15) 19.3
  Ano 13 Pre Yes 2.28 × 105 (0.54) 23.5
  Ano 25 Back No 14.99 × 105 (0.45) 3.0
  Ano 25 Back Yes 3.22 × 105 (1.06) 32.9
Southeastern US coastal waters Ano 25 Pre No 4.41 × 105 (0.62) 13.9
  Ano 25 Pre Yes 3.28 × 105 (0.35) 10.7
  Ano 13 Pre No 2.58 × 105 (0.35) 13.7
  Ano 13 Pre Yes 2.75 × 105 (0.41) 14.9
  1. a Anodisc™ 25 mm (Ano 25) and 13 mm (Ano 13) membranes
  2. b Average VLP abundance from triplicate filters along with the standard deviation
  3. c The percent coefficient of variation from 3 replicate measures.