Table 1 Bacterial strains and plasmids used in this study
Strain/plasmid | Genotype or relevant characteristics | Origin |
|---|---|---|
C. jejunistrains | ||
81-176 | parental strain; pVir, pTet (TetR) | G. Perez - Perez * |
AG1 | 81-176 dba::aphA-3 | This study |
AL1 | 81-176 dsbI::cat | This study |
AG6 | 81-176 Δdba-dsbI::cat | This study |
AG11 | 81-176 fur::cat | This study |
480 | parental strain | J. van Putten ** |
AL4 | 480 dsbI::cat | This study |
AG15 | 480 fur::cat | This study |
E. coli strains | ||
DH5α | F- Φ80d lacZ ΔM15 Δ(lacZYA-orgF)U169 deoR recA1endA1 hsdR17 (rk- mk+) phoA supE44 λ- thi-1 gyrA96 relA1 | Gibco BRL |
TG1 | supE44 hsdΔ 5 thi Δ(lac- proAB) F' [traD36 proAB + lacIq lacZΔM15] | [26] |
S17-1 | recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 Tmpr, Spcr, Strr | [56] |
General cloning/Plasmid vectors | ||
pGEM-T Easy | Apr; LacZα | Promega |
pRY107 | Kmr; E. coli/C. jejuni shuttle vector | [27] |
pRY109 | Cmr; E. coli/C. jejuni shuttle vector | [27] |
pRK2013 | Kmr; helper vector for E. coli/C. jejuni conjugation | [28] |
Plasmids for gene expression study Cj stands for PCR-amplified C. jejuni 81-176 DNA fragment (PCR primers are given in brackets) Cc stands for PCR-amplified C.coli 72Dz/92 DNA fragment (PCR primers are given in brackets) cj stands for C. jejuni 81-176 gene | ||
pUWM471 | pMW10/1300 bp Cc (H0B - H4X) | [39] |
pUWM803 | pMW10/440 bp Cj (Cjj879B - Cjj880X) | This study |
pUWM792 | pMW10/1170 bp Cj (Cjj879B - Cjj881X) | This study |
pUWM795 | pMW10/1980 bp Cj (Cjj879B - Cjj882X) | This study |
pUWM832 | pMW10/690 bp Cj (Cjj880B - Cjj880X) | This study |
pUWM833 | pMW10/750 bp Cj (Cjj880B2 - Cjj881X) | This study |
pUWM834 | pMW10/900 bp Cj (Cjj881B - Cjj882X) | This study |
pUWM864 | pMW10/660 bp Cj (Cjj882B3 - Cjj883X2) | This study |
pUWM827 | pMW10/540 bp Cj (Cj19LX-2 - Cj18Bgl) | This study |
pUWM828 | pMW10/720 bp Cj (Cj19LX-2 - Cj17Bgl) | This study |
pUWM858 | pMW10/240 bp Cj (Cjj45B - Cjj44X) | This study |
Plasmids for mutagenesis | ||
pAV80 | pBluescript II SK/cjfur::cat | [25] |
pUWM622 | pBluescript II KS/cjdba::aphA-3 | This study |
pUWM713 | pGEM-T Easy/cjdsbI::cat | This study |
pUWM867 | pGEM-T Easy/Δcjdba-cjdsbI::cat | This study |
Plasmids for translational coupling study | ||
pUWM769 | pRY107/cjdba-cjdsbI operon | This study |
pUWM811 | pRY107/cjdba (M1R)-cjdsbI operon | This study |
pUWM812 | pRY107/cjdba (L29stop)-cjdsbI operon | This study |
pUWM1072 | pBluescript II SK/promoter of cjdba-cjdsbI operon | This study |
pUWM1100 | pBluescript II SK/cjdsbI with its own promoter | This study |
pUWM1103 | pRY107/cjdsbI with its own promoter | This study |
Plasmid for recombinant protein synthesis and purification | ||
pUWM657 | pET28a/cjdsbI (1100 bp 5'-terminal fragment) | This study |
pUWM1098 | pET24d/cjfur (fur coding region) | This study |