Analysis of C. jejuni dsbI transcription from a dba-dsbI operon containing wild type or point mutated dba. RT-PCR analysis of dsbI (and aphA-3) transcription in C. jejuni wild type and mutant cells. Equal amounts of mRNAs isolated from C. jejuni cells were reverse-transcribed using primer KM-R1 or Cj-RT and resulting cDNA was PCR-amplified with primer pairs KM-L1 - KM-R1 (lanes 1-7) or CjNde - Cj17RM (lanes 8-14), respectively. Relative positions of DNA molecular length markers (lanes 1, 8) are listed on the left (in base pairs). Lanes 2-6 and 9-13 contain PCR products amplified on cDNAs for C. jejuni 81-176 wt (2, 9), AG6 (dba-dsbI::cat) (3, 10), AG6/pUWM811 (4, 11), AG6/pUWM812 (5, 12), AG6/pUWM769 (6, 13); lanes 7 and 14 contain PCR products amplified on RNA for AG6/pUWM769 (after DNase treatment). White arrows indicate products of expected size.