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Figure 3 | BMC Microbiology

Figure 3

From: Campylobacter jejuni dsb gene expression is regulated by iron in a Fur-dependent manner and by a translational coupling mechanism

Figure 3

Electrophoretic mobility shift assays of chuA, dba-dsbI, dsbA2 and dsbA1 promoter regions bound by CjFur-His 6 . 28 fmol of Dig-labelled PCR amplified DNA fragments: dsbA2 (333 bp - panel A and B), dsbA1 (299 bp- panel C and D), dba-dsbI (174 bp - panel E and F) and chuA (216 bp- panel G and H) were incubated with 0, 333, 1000 or 3333 nM of purified Fur protein. The concentration of CjFur-His6 used in the reactions is indicated above the lanes. Binding buffer used in four EMSA studies (panels B, D, F, H) does not contain Mn2+. Panel I presents competition gel mobility shift assay which was performed by incubation of 3333 nmol Fur-His protein with 28 fmol of the labelled promoter region upstream of dsbA2-dsbB-astA operon (dsbA2) and various concentrations of the unlabelled promoter region upstream of dsbA2-dsbB-astA operon (dsbA2*)

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