Figure 2

Transcription levels of C. jejuni 81-76 dsb genes (measured by β-galactosidase activity assays) in the wild type strain (A and B) and fur::cat mutant (C) under different environmental conditions. Each experiment was repeated three times, and each time three independent samples were taken for each strain (giving 9 independent measurements for each strain). Statistical significance was calculated using t-Student test for comparison of independent groups (GraphPad Prism). The wild type strain C. jejuni 480 carrying an empty vector pMW10 was used as a control.

Statistical p values:

For wild type C. jejuni 480 strain:

P _dba-dsbI temp. 37°C vs 42°C: p = 0,0001(*).

P _{dsbA2-dsbB-astA} temp. 37°C vs 42°C: p = 0,2020.

P _dsbA1 temp. 37°C vs 42°C: p = 0,1031.

P _dba-dsbI MH+Fe vs MH: p = 0,0576.

P _dba-dsbI MH-Fe vs MH: p < 0,0001(*).

P _{dsbA1-dsbB-astA} MH+Fe vs MH: p = 0,0007(*).

P _{dsbA1-dsbB-astA} MH-Fe vs MH: p < 0,0001(*).

P _dsbA1 MH+Fe vs MH: p = 0,2569.

P _dsbA1 MH-Fe vs MH: p < 0,0001(*).

For mutant C. jejuni 480 fur::cat strain:

P _dba-dsbI MH+Fe vs MH: p = 0,3683.

P _dba-dsbI MH-Fe vs MH: p = 0,6796.

P _{dsbA1-dsbB-astA} MH+Fe vs MH: p = 0,3164.

P _{dsbA1-dsbB-astA} MH-Fe vs MH: p = 0,0577.

P _dsbA1 MH+Fe vs MH: p = 0,5228.

P _dsbA1 MH-Fe vs MH: p = 0,2388.

P values of P < 0.05 were considered to be statistically significant; they are marked with (*).