Figure 2
From: Electron transport in acetate-grown Methanosarcina acetivorans
Ferredoxin:heterodisulfide oxidoreductase activity of membranes. The 100-μl reaction mixture consisted of 20 mM NADPH, 2 μg FNR (Sigma), the membrane fraction of acetate-grown cells (60 μg protein), 1.1 mM CoM-S-S-CoB and the indicated concentrations of ferredoxin contained in 50 mM MOPS buffer (pH 6.8). Total thiols were determined by the DTNB assay. Symbols: (filled triangles)1.2 μM ferredoxin, (filled circles) 0.6 μM ferredoxin, (filled squares) 0.3 μM ferredoxin, (open circles) minus ferredoxin.