Figure 1

Reduction of ferredoxin by CdhAE. The 70-μl reaction mixture consisted of 2.2 μg of CdhAE and 28 μM (final concentration) of ferredoxin contained in 50 mM MOPS buffer (pH 6.8) under 1 atm CO. The reaction was initiated with CdhAE. A, complete reaction mixture initial absorbance 0.61. B, reaction mixture minus CdhAE, initial absorbance 0.72. C, reaction mixture minus ferredoxin, initial absorbance 0.72. The reduction of ferredoxin was followed by the decrease in absorbance at 402 nm.