Table 2 Strains and plasmids used in this study
Strains or plasmids | relevant characteristics | Source or reference |
|---|---|---|
Strains | Â | Â |
S. ansochromogenes 7100 | Wild-type strain | [40] |
sabRDM | The sabR disruption mutant | [24] |
E. coli DH5α | F- recA f80 dlacZ ΔM15 | Gibco BRL |
BL21(DE3) | F- ompT hsdS gal dcm (DE3) | Novagen |
ET12567 (pUZ8002) | recE dam dcm hsdS Cmr Strr Tetr Kmr | [41] |
Alternaria longipes | Indicator strain for nikkomycin bioassays | [40] |
Plasmids | Â | Â |
pBluescript KS+ | Routine cloning and subcloning vector | Stratagene |
pET23b | Expression vector | Novagen |
pET23b::sabR | sabR gene cloned in pET23b | This work |
pIJ8600 | ori pUC, oriT RK2, int ΦC31, tipAp, tsr, aprR | [33] |
pIJ8600::sabR | sabR gene cloned in the induced vector of pIJ8600 which containing PtipA as promoter | This work |
pKC1139 | E.coli-Streptomyces shuttle vector | [33] |
pGARE1 | A 974 bp DNA fragment containing the left flank of SARE was inserted into pUC119::kan | This work |
pGARE2 | A 806 bp DNA fragment containing the right flank of SARE was inserted into GAREL1 | This work |
pGARE3 | A 2.8 kb DNA fragment containing the left and right flanks of SARE and kanamycin resistance gene from pGARE2 was inserted into pKC1139 | This work |
pGARE4 | The 1 kb kanamycin resistance gene was deleted from pGARE3 | This work |
pGARE5 | A 1.8 kb DNA fragment containing the left and right flanks of SARE from pGARE4 was inserted into pKC1139 | This work |