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Table 1 Primers used in this study

From: SabR enhances nikkomycin production via regulating the transcriptional level of sanG, a pathway-specific regulatory gene in Streptomyces ansochromogenes

Gene and primer

Positions*

Sequence (5'-3')

sabR

  

sab1-F

+38 to +57

CATATGGCTCAGCAGGACCGAGC

sab1-R

+697 to +681

CTCGAGGCAGGCGATGCCCGACA

sab2-F

+38 to +57

CATATGGCTCAGCAGGACCGAGC

sab2-R

+700 to +681

GGATCCTCAGCAGGCGATGCCCGACA

ER-F

-132 to -115

CCCCGTGGCACCGTTCAT

ER-R

+143 to +160

GACACCGGCCCGCTTGAG

S1R-F

-457 to -440

GCCAGAGCCGACACCACA

S1R-R

+44 to +60

ACGGCTCGGTCCTGCTG

sanG

  

Gare1-F

-1035 to -1016

GGAATTCCGCCCGGCAGCAGCTGGACT

Gare1-R

-62 to -85

GGGGTACCAAGTGGCTCCATGTATCCGCGACC

Gare2-F

-34 to -12

GCTCTAGACCAGCTCAGGAGAATGCTCGATA

Gare2-R

+772 to +753

CCCAAGCTTCTCCGCGACCTCGTCATCAT

EG0-F

+1404 to +1423

AGGCCACCCTGCAGACGTAC

EG0-R

+1741 to +1724

GAGGAGCGTGTCGGCTTG

EG1-F

-415 to -396

GCGGGAGAACGTCACCTGTT

EG1-R

+104 to +86

CGGGTCGGCTGTGGTGAGT

EG2-F

+340 to +360

GCTCCGAGACCGTGACGAAAG

EG2-R

+763 to +743

CTCGTCATCATCAGCGTGGGT

EG3-F

+743 to +763

ACCCACGCTGATGATGACGAG

EG3-R

+1218 to +1198

GACGCGGTTTGTTGCTCTTGA

RealG-F

+1405 to +1423

GGCCACCCTGCAGACGTAC

RealG-R

+1536 to +1519

CGGGACAGGTCGAACGTG

sanN and sanO

  

ENO-F

-114 to -98

TCTTGGTCGCCAGGTCC

ENO-R

+135 to +153

CTTCGGATGCTGAATGTGC

sanF

  

EF-F

-267 to -250

CGCGCAGGTCGGCCAGGT

EF-R

+234 to +213

TACTGCTTCTCGTGCTTCGGGT

RealF-F

+70 to +87

GGTGCTGACGCTCGACTC

RealF-R

+257 to +238

TGAGGTCCACGAGGTTCATC

hrdB

  

S1H-F

-820 to -803

GGGTACGCCCCGTCAGTG

S1H-R

+241 to +224

AGCCTTTCCCCGCTCAAT

RealH-F

+290 to +308

ACTGAGTGGCCGGAATCTG

RealH-R

+225 to +206

GTCTCTGTCATGGCGCTCAT

  1. * The nucleotide positions for sanG, sanN, sanO, sanF, sabR and hrdB are given with respect to their transcription start points as +1. The enzyme recognition sites are underlined in the primer sequence.