Skip to main content
Figure 2 | BMC Microbiology

Figure 2

From: SabR enhances nikkomycin production via regulating the transcriptional level of sanG, a pathway-specific regulatory gene in Streptomyces ansochromogenes

Figure 2

Effects of over-expresson of sabR on nikkomycin biosynthesis and morphological differentiation of S. ansochromogenes. A, Nikkomycin bioassay of fermentation filtrates from different strains with induction of thiostrepton (the left side) or without induction of thiostrepton as control (the right side). Thiostrepton (10 μg ml-1) was added to the cultures after incubation for 12 h in SP medium. B, Phenotype of the sabR overexpressed strain (8600R) with induction of thiostrepton (the left side) or without induction of thiostrepton as control (the right side). Thiostrepton (10 μg ml-1) was added to the medium. C, Scanning electron micrographs of 8600R and 8600 which were grown at 28°C for 96 h in different media. MMM, MMG and MS media supplemented with thiostrepton (10 μg ml-1) were used. 8600, the wild-type strain carrying pIJ8600. MMM, minimal medium (MM) containing mannitol (0.5 %, w/v) as carbon source; MMG, MM containing glucose (1 %, w/v) as carbon source; MS, Mannitol soya flour medium.

Back to article page