Table 1 Bacterial strains and plasmids

L. monocytogenes

EGD-e

ΔtrpS × pFlo-trpS

wild-type

T. Chakraborty (University of Giessen, Germany [36]

ΔtrpS,inlA/B × pFlo-trpS

[32]

Lm-spa^-

ΔtrpS,aroA,inlA/B × pFlo-trpS

This work

Lm-spa⁺

ΔtrpS,aroA,inlA/B,int::P_hly-spa × pFlo-trpS

This work

ΔtrpS × pSP0-P_actA-gfp

[36]

Lm-spa^- × pSP0-P _actA -gfp

ΔtrpS,aroA,inlA/B × pSP0-P_actA-gfp

This work

Lm-spa⁺,aroA⁺ × pSP0-P _actA -gfp

ΔtrpS,inlA/B,int::P_hly-spa × pSP0-P_actA-gfp

This work

Lm-spa⁺ × pSP0-P _actA -gfp

ΔtrpS,aroA,inlA/B,int::P_hly-spa × pSP0-P_actA-gfp

This work

Plasmids

pFlo-trpS

Tc^R,

[36]

pSP0-P_actA-gfp

Em^R, gfp-ORF, actA-promoter

[36]

pLSV101intAB

Em^R, ORI^ts, mutagenesis plasmid

[31]

pLSV101intAB::P _hly -spa

spa-ORF, hly-promoter

This work