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Figure 4 | BMC Microbiology

Figure 4

From: Specific antibody-receptor interactions trigger InlAB-independent uptake of listeria monocytogenes into tumor cell lines

Figure 4

Effect of serum incubation on antibody-mediated internalization of Lm-spa+. The bacteria were incubated with PBS (-mAb), Cetuximab or Trastuzumab and the antibodies were covalently bound to protein A by crosslinking with DMP. Subsequently the bacteria were incubated with murine serum prior to infection of 4T1-HER2 cells. Intracellular CFU was determined after gentamicin treatment by plating serial dilutions. The relative internalization rate in comparison to uncoated bacteria was calculated and is shown.

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BMC Microbiology

ISSN: 1471-2180

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