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Figure 3 | BMC Microbiology

Figure 3

From: Calcium/calmodulin kinase1 and its relation to thermotolerance and HSP90 in Sporothrix schenckii: an RNAi and yeast two-hybrid study

Figure 3

Analysis of the expression of sscmk1 RNA in S. schenckii cells transformed with pSD2G or pSD2G-RNAi1 grown at 35°C and 25°C. The expression of sscmk1 gene RNA was determined in cells transformed with plasmid pSD2G and plasmid pSD2G-RNAi1. RNA was extracted as described in Methods from cells growing in a modification of medium M with geneticin (500 μg/ml) at 35°C or cells growing in a modification of medium M with geneticin (500 μg/ml) at 25°C. A minimum of 3 independent experiments were performed for each transformant. The average ± the standard deviation of the ng of sscmk1 RNA/ng of total RNA was calculated using the standard curve. The Student's T test was used to determine the significance of the data (p < 0.05). Results significantly different from the control values are marked with an asterisk.

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