Figure 2

Macroscopic and microscopic appearance of S. schenckii transformants and controls incubated at 35°C and 25°C. Figures 2A and 2B show the appearance of S. schenckii transformed with pSD2G, pSD2G-RNAi1 or pSD2G-RNAi2 grown in liquid medium w/wo geneticin (500 μg/ml) and incubated at 35°C. In Figure 2A, tube 1 shows the growth of the wild type cells (no geneticin added to the medium), tube 2 shows the growth of cells transformed with the empty plasmid (pSD2G). Tubes 3 to 7 show the growth obtained from colonies 19, 21, 29, 33 and 47, respectively that were transformed with pSD2G-RNAi1. In Figure 2B, tubes 1 and 2 show the growth observed with the wild type cells and cells transformed with the pSD2G, respectively. Tubes 3 to 6 show the growth obtained from colonies 1, 2, 7 and 16, transformed with pSD2G-RNAi2. Figure 2C, 2D and 2E show the appearance of S. schenckii transformed with pSD2G or pSD2G-RNAi1 grown in solid medium w/wo geneticin (500 μg/ml) and incubated at 25°C. Figure 2C shows the growth of cells transformed with pSD2G. Figure 2D and 2E show the growth obtained from colonies 19 and 21 transformed with pSD2G-RNAi1, respectively. Figure 2F, 2G and 2H show the microscopic morphology of wild type and transformed cells of S. schenckii grown from conidia as described in Methods for 5 days at 35°C in liquid medium w/wo geneticin (500 μg/ml) and mounted on lactophenol cotton blue. Samples F and G correspond to the wild type and cells transformed with pSD2G respectively, at 40× magnification. Sample H shows the appearance of cells transformed with the sscmk1 pSD2G-RNAi1 at 20× magnification. Figure 2I and 2J show the microscopic morphology on slide cultures of S. schenckii grown from conidia as described in Methods at 25°C in solid medium w/wo geneticin (500 μg/ml) and mounted on lactophenol cotton blue of cells transformed with pSD2G and cells transformed with pSD2G-RNAi1, respectively.