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Figure 2 | BMC Microbiology

Figure 2

From: Identification of two linear B-cell epitopes from West Nile virus NS1 by screening a phage-displayed random peptide library

Figure 2

Reactivity of mAbs with recombinant NS1 and C6/36 cells infected with flaviviruses. (a) Western blot analysis of mAbs 3C7 (lanes 1, 2) and 4D1 (lanes 3, 4) against recombination NS1 (lane 1, 3) and MBP-tag (lane 2, 4). M, PageRuler™ Prestained Protein Ladder (Fermentas, Canada). (b) Pattern of immunofluorescence produced by anti-NS1 mAbs on antigen slides which were prepared on porous slides using C6/36 cells infected with different flaviviruses. Panels 1-8: reactivity of mAb 3C7 with cells infected with WNV (panel 1), JEV (panel 2), DENV1 (panel 3), DENV2 (panel 4), DENV3 (panel 5), DENV4 (panel 6), YFV (panel 7), and TBEV (panel 8). Panels 11-18: reactivity of mAb 4D1 with cells infected with WNV (panel 11), JEV (panel 12), DENV1 (panel 13), DENV2 (panel 14), DENV3 (panel 15), DENV4 (panel 16), YFV (panel 17), and TBEV (panel 18). Panels 9 and 19 are positive controls which used WNV-positive mouse serum. Panels 10 and 20 are negative controls which used WNV-negative mouse serum.

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BMC Microbiology

ISSN: 1471-2180

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