Skip to main content

Advertisement

Table 1 Strains and plasmids

From: Induction kinetics of the Staphylococcus aureus cell wall stress stimulon in response to different cell wall active antibiotics

Strain/plasmid Relevant genotypea Reference/source
S. aureus
RN4220 Restriction-negative derivative of NCTC8325-4 [48]
BB255 NCTC8325 derivative, cured of plasmid pI524 [49]
BB255ΔVraR BB255 containing vraR mutation, truncating VraR after the 2nd amino acid This study
E. coli
DH5α F- φ80lac Z∆M15 ∆(lac ZYA-arg F)U169 rec A1 end A1 hsd R17(rk-, mk+) pho A sup E44 thi-1 gyr A96 rel A1λ- Invitrogen
Plasmids
pSP-luc+ Luciferase fusion plasmid, ori ColE1, bla, luc+; Apr Promega
pBUS1 E. coli - S. aureus shuttle vector, tetL ; Tcr [31]
pKOR1 E. coli - S. aureus shuttle plasmid, for creating markerless deletions; repF (ts), cat, attP, ccdB, ori ColE1, bla, Pxyl/tetO, secY570; Apr, Cmr [25]
pKOR1-VraR::stop pKOR1 construct containing mutant vraR insert with XhoI site and two inframe stop codons inserted between the 2nd and 3rd vraR codons. [26]
psas016p-luc+ pBUS1 containing the sas016 promoter-luciferase reporter gene fusion [26]
ptcaAp-luc+ pBUS1 containing the tcaA promoter-luciferase reporter gene fusion This study
psa0908p-luc+ pBUS1 containing the sa0908 promoter-luciferase reporter gene fusion This study
  1. aAbbreviations: Tcr, tetracycline resistance; Apr, ampicillin resistance; Cmr, chloramphenicol resistance