S. aureus
|
RN4220
|
Restriction-negative derivative of NCTC8325-4
|
[48]
|
BB255
|
NCTC8325 derivative, cured of plasmid pI524
|
[49]
|
BB255ΔVraR
|
BB255 containing vraR mutation, truncating VraR after the 2nd amino acid
|
This study
|
E. coli
|
DH5α
|
F- φ80lac Z∆M15 ∆(lac ZYA-arg F)U169 rec A1 end A1 hsd R17(rk-, mk+) pho A sup E44 thi-1 gyr A96 rel A1λ-
|
Invitrogen
|
Plasmids
|
pSP-luc+
|
Luciferase fusion plasmid, ori ColE1, bla, luc+; Apr
|
Promega
|
pBUS1
|
E. coli - S. aureus shuttle vector, tetL ; Tcr
|
[31]
|
pKOR1
|
E. coli - S. aureus shuttle plasmid, for creating markerless deletions; repF (ts), cat, attP, ccdB, ori ColE1, bla, Pxyl/tetO, secY570; Apr, Cmr
|
[25]
|
pKOR1-VraR::stop
|
pKOR1 construct containing mutant vraR insert with XhoI site and two inframe stop codons inserted between the 2nd and 3rd vraR codons.
|
[26]
|
psas016p-luc+
|
pBUS1 containing the sas016 promoter-luciferase reporter gene fusion
|
[26]
|
ptcaAp-luc+
|
pBUS1 containing the tcaA promoter-luciferase reporter gene fusion
|
This study
|
psa0908p-luc+
|
pBUS1 containing the sa0908 promoter-luciferase reporter gene fusion
|
This study
|