Induction kinetics of the Staphylococcus aureus cell wall stress stimulon in response to different cell wall active antibiotics

Table 1 Strains and plasmids

Strain/plasmid	Relevant genotype^a	Reference/source
*S. aureus*
RN4220	Restriction-negative derivative of NCTC8325-4	[48]
BB255	NCTC8325 derivative, cured of plasmid pI524	[49]
BB255ΔVraR	BB255 containing vraR mutation, truncating VraR after the 2^nd amino acid	This study
*E. coli*
DH5α	F^- φ80lac Z∆M15 ∆(lac ZYA-arg F)U169 rec A1 end A1 hsd R17(r_k^-, m_k⁺) pho A sup E44 thi-1 gyr A96 rel A1λ^-	Invitrogen
Plasmids
pSP-luc+	Luciferase fusion plasmid, ori ColE1, bla, luc+; Ap^r	Promega
pBUS1	E. coli - S. aureus shuttle vector, tetL ; Tc^r	[31]
pKOR1	E. coli - S. aureus shuttle plasmid, for creating markerless deletions; repF (ts), cat, attP, ccdB, ori ColE1, bla, Pxyl/tetO, secY570; Ap^r, Cm^r	[25]
pKOR1-VraR::stop	pKOR1 construct containing mutant vraR insert with XhoI site and two inframe stop codons inserted between the 2^nd and 3^rd vraR codons.	[26]
psas016_p-luc+	pBUS1 containing the sas016 promoter-luciferase reporter gene fusion	[26]
ptcaA_p-luc+	pBUS1 containing the tcaA promoter-luciferase reporter gene fusion	This study
psa0908_p-luc+	pBUS1 containing the sa0908 promoter-luciferase reporter gene fusion	This study

^aAbbreviations: Tc^r, tetracycline resistance; Ap^r, ampicillin resistance; Cm^r, chloramphenicol resistance

ISSN: 1471-2180