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Table 1 Bacterial strains and plasmid used in this work

From: The replication origin of a repABC plasmid

Strain Relevant characteristics Reference
Rhizobium etli CE3 Streptomycin resistant derivative of CFN42 strain [20]
R. etli CFNX101 recA::Ω-Spectinomycin derivative of CE3 [46]
R. etli CFNX107 recA:: Ω-Spectinomycin derivative of CE3, laking plasmid p42a and p42d. [46]
E. coli S17-1 Plasmid donor in conjugations [23]
Plasmid Relevant characteristics Reference
pDOP A chloramphenicol resistant suicide vector derived from pBC SK(+), and containing oriT This work
pDOP-E' pDOP derivative with the intergenic region repB-repC, the complete repC gene under Placpromoter, and 500 pb downstream repC stop codon. [22]
pDOP-H3 pDOP derivative carrying a 5.6 Kb HindIII with repABC operon of R. etli plasmid p42d. This work
pDOP-αC pDOP derivative with the intergenic region repB-repC and the complete repC gene under Plac promoter. This work
pDOP-C pDOP carrying repC gen of plasmid p42d, with a SD sequence (AGGA) and under Plac promoter. This work
pDOP-C/D1UM Similar to pDOP-C but with a repC gene carrying a deletion from codon 2 to codon 29 This work
pDOP-C/RD1L Similar to pDOP-C but with a repC gene carrying a deletion from codon 372 to codon 401 This work
pDOP-F1 pDOP containing a repC fragment from codon 2 to codon 110, with a SD consensus sequence under Plac promoter. This work
pDOP-C/F1-F2 pDOP containing a repC fragment from codon 2 to codon 209, with a SD consensus sequence under Plac promoter. This work
pDOP-C/F1-F3 pDOP containing a repC fragment from codon 2 to codon 309, with a SD consensus sequence under Plac promoter. This work
pDOP-C/F4 pDOP containing a repC fragment from codon 310 to codon 403, with a SD consensus sequence under Plac promoter. This work
pDOP-C/F4-F3 pDOP containing a repC fragment from codon 210 to codon 403, with a SD consensus sequence under Plac promoter. This work
pDOP-C/F4-F2 pDOP containing a repC fragment from codon 111 to codon 403, with a SD consensus sequence under Plac promoter. This work
pDOP-C s/SD Similar to pDOP-C but without the SD sequence This work
pDOP-TtMC Similar to pDOP-C but with a mutant repC gene carrying This work
  silent mutations to increase its CG content  
pDOP-CBbglll Similar to pDOP-C but with repC gene, carrying a frameshift mutation at the BglII restriction site This work
pDOP-CSphI Similar to pDOP-C but with repC gene, carrying a frameshift mutation at the SphI restriction site This work
pDOP-CAtLC pDOP derivative carrying repC gen of the Agrobacterium This work
  tumefaciens C58 linear chromosome, with a SD sequence (AGGA) and under Plac promoter.  
pDOP-CsA pDOP derivative carrying repC gen of the Sinorhizobium meliloti 1021 pSymA, with a SD sequence (AGGA) and under Plac promoter. This work
pDOP/C420-1209 pDOP with a hybrid repC gene, encoding the first 140 amino acid residues of the pSymA RepC protein and the rest of p42d. This work
pDOP/C1-420 pDOP with a hybrid repC gene, encoding the first 140 amino acid residues of the p42d RepC protein and the rest of pSymA. This work
pDOP/C421-840 pDOP with a hybrid repC gene encoding, the first 140 amino acid residues of the pSymA RepC protein, the next 140 amino acid residues from the p42d RepC protein and the rest from the pSymA RepC protein. This work
pDOP/Cs421-840 pDOP witha hybrid repC gene, encoding the first 140 amino acid residues of the p42d RepC protein, the next 140 amino acid residues from the pSymA RepC protein and the rest from the p42d RepC protein. This work
pDOP/C841-1209 pDOP derivative with a hybrid repC gene, encoding the first 280 aminoacid residued of pSymA RepC and the rest of p42d RepC protein. This work
pDOP/C1-990 pDOP derivative with a hybrid repC gene, encoding the first 330 amino acid residues of p42d RepC protein and the rest of pSymA RepC protein. This work
pDOP/C1-1086 pDOP derivative with a hybrid repC gene, encoding the first 362 amino acid residues of p42d RepC protein and the rest of pSymA RepC protein. This work