Table 5 Sequences of the primers used for the construction of a full-length cDNA clone and mutants of FMDV Asia1/JSp1c8
Name | Nucleotide Sequence (5'→3') | Nucleotide Position (nt) |
|---|---|---|
E1 | CAGGATCC TAATACGACTCACTATAGGGTTGAAAAGG GGCGCTAGGGTC | 1-21 |
E1' | TAAAACTTAGGGGGGGGGGGGGGGGGGGGTGAAAG | 361-390 |
E2 | TTTCACCCCCCCCCCCCCCCCCCCCTAAGTTTTAC | 362-391 |
E2' | CCTCTAGA CCTGGAAAGACCAGGC | 677-700 |
E3 | AGGTCTAGAGGGGTGACATTTTGT | 690-713 |
E3' | GTCTGCAGCAGAAAGGTAAGGGAT | 3078-3101 |
E4 | CTGCTGCAGACTATGCTTACACTG | 3090-3113 |
E4' | AAAGAATTC AATTGCTGCCTCATG | 5414-5437 |
E5 | AATTGAATTCTTTGAGGGAATGGTGCAC | 5425-5452 |
E5' | TTGCGGCCGCTTT(38) | 3'end |
P1 | ACAAGGAAAGATGGAGCTCACACTTCACAAC | 1168-1198 |
P1' | GTTGTGAAGTGTGAGCTCCATCTTTCCTTGT | 1168-1198 |
TR1 | ACTGCATTCATTCTGAGTGGGA | 2960-2984 |
TR1' | GGCAAGATCACCACGCCGCGAGGA | 3679-3703(D→G) |
TR2 | TCCTCGCGGCGTGGTGATCTTGCC | 3679-3703(D→G) |
TR2' | 5'-GAAGAAACTCGAGGCGACTTTGAC-3' | 4342-4366 |
TR3 | TCCTCGCGGCGTAGTGATCTTGCC | 3679-3703(D→S) |
TR3' | GGCAAGATCACTACGCCGCGAGGA | 3679-3703(D→S) |