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Table 1 MLST Primers and PCR Primers used in the amplification of the genes and the expected product sizes used in this study.

From: Molecular and comparative analysis of Salmonella entericaSenftenberg from humans and animals using PFGE, MLST and NARMS

Gene PCR Primers Product size Gene Sequencing Primers
thrA: F 5'-GTCACGGTGATCGATCCGGT-3' 852 bp thrA: sF 5'-ATCCCGGCCGATCACATGAT-3'
thrA: R 5'-CACGATATTGATATTAGCCCG-3'   thrA: sR 5'-CTCCAGCAGCCCCTCTTTCAG-3'
thrA: R1 5'-GTGCGCATACCGTCGCCGAC-3' (also Seq)   
purE: F 5'-ATGTCTTCCCGCAATAATCC-3' 510 bp purE: sF 5'-CGCATTATTCCGGCGCGTGT-3'
purE: R 5'-TCATAGCGTCCCCCGCGGATC-3'   purE: sF1 5'-CGCAATAATCCGGCGCGTGT-3'
purE: R1 5'-CGAGAACGCAAACTTGCTTC-3'   purE: sR 5'-CGCGGATCGGGATTTTCCAG-3'
   purE: sR1 5'-GAACGCAAACTTGCTTCAT-3'
sucA: F 5'-AGCACCGAAGAGAAACGCTG-3' 643 bp sucA: sF 5'-AGCACCGAAGAGAAACGCTG-3'
sucA: R 5'-GGTTGTTGATAACGATACGTAC-3'   sucA: sR 5'-GGTTGTTGATAACGATACGTAC-3'
hisD: F 5'-GAAACGTTCCATTCCGCGCAGAC-3' 894 bp hisD: sF 5'-GTCGGTCTGTATATTCCCGG-3'
hisD: R 5'-CTGAACGGTCATCCGTTTCTG-3'   hisD: sR 5'-GGTAATCGCATCCACCAAATC-3'
aroC: F 5'-CCTGGCACCTCGCGCTATAC-3' 826 bp aroC: sF 5'-GGCACCAGTATTGGCCTGCT-3'
aroC: R 5'-CCACACACGGATCGTGGCG-3'   aroC: sR 5'-CATATGCGCCACAATGTGTTG-3'
hemD: F 5'-ATGAGTATTCTGATCACCCG-3' 666 bp hemD: sF 5'-GTGGCCTGGAGTTTTCCACT-3'
hemD: F1 5'-GAAGCGTTAGTGAGCCGTCTGCG-3'   hemD: sF1 5'-ATTCTGATCACCCGCCCCTC-3'
hemD: R 5'-ATCAGCGACCTTAATATCTTGCCA-3'   hemD: sR 5'-GACCAATAGCCGACAGCGTAG-3'
dnaN: F 5'-ATGAAATTTACCGTTGAACGTGA-3' 833 bp dnaN: sF 5'-CCGATTCTCGGTAACCTGCT-3'
dnaN: R 5'-AATTTCTCATTCGAGAGGATTGC-3'   dnaN: sR 5'-CCATCCACCAGCTTCGAGGT-3'
dnaN: R1 5'-CCGCGGAATTTCTCATTCGAG-3' (also Seq)