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Table 1 Strains and plasmids used in this study

From: The conjugative plasmid of a bean-nodulating Sinorhizobium fredii strain is assembled from sequences of two Rhizobium plasmids and the chromosome of a Sinorhizobiumstrain

Strain Relevant characteristic Source
CFN42 wild type R. etli (pRet42a to pRet42f) [58]
CFN2001 CFN42 lacking pRet42a and pRet42d [37]
CFNX195 CFN42 derivative cured of pRet42a, pRet42d::Tn5mob [32]
GR64 wild type bean-nodulating S. fredii (pSfr64a, pSfr64b) [12]
GR64-1 GR64/pSfr64a::Tn5-GDYN, pSfr64b::Tn5mob This work
GR64-2 GR64 cured of pSfr64a, pSfr64b::Tn5mob This work
GR64-3 GR64-2 with pRet42a::Tn5-GDYN This work
GR64-4 GR64 cured of pSfr64a and pSfr64b, RifR This work
GR64-5 GR64-4/pRet42a::Tn5-GDYN This work
GR64-6 GR64-4/pSfr64a::Tn5-GDYN This work
CFN2001-1 CFN2001/pSfr64b::Tn5mob This work
CFN2001-2 CFN2001/pSfr64b::Tn5mob, pRet42a::Tn5-GDYN This work
CFN2001-3 CFN2001/pSfr64b::Tn5mob, pSfr64a::Tn5-GDYN This work
Escherichia coli   
DH5a Receptor for transformation [59]
S17-1 C600::RP-4-2 (Tc::Mu)(Km::Tn7) [60]
S17/pDR21 Source of Tn5-GDYN [17]
Agrobacterium tumefaciens   
GMI9023 C-58 cured of its native plasmids [35]
UIA143 recA pTi- derivative of C58 [61]
GMI9023/pSfr64a GMI9023 with pSfr64a::Tn5-GDYN This work
GMI9023/pSfr64b GMI9023 with pSfr64b::Tn5mob This work
pSUP5011 Tn5mob [60]
pRK2013 Conjugation helper [36]