The conjugative plasmid of a bean-nodulating Sinorhizobium fredii strain is assembled from sequences of two Rhizobium plasmids and the chromosome of a Sinorhizobiumstrain

Table 1 Strains and plasmids used in this study

Strain	Relevant characteristic	Source
*Rhizobium*
CFN42	wild type R. etli (pRet42a to pRet42f)	[58]
CFN2001	CFN42 lacking pRet42a and pRet42d	[37]
CFNX195	CFN42 derivative cured of pRet42a, pRet42d::Tn5mob	[32]
GR64	wild type bean-nodulating S. fredii (pSfr64a, pSfr64b)	[12]
GR64-1	GR64/pSfr64a::Tn5-GDYN, pSfr64b::Tn5mob	This work
GR64-2	GR64 cured of pSfr64a, pSfr64b::Tn5mob	This work
GR64-3	GR64-2 with pRet42a::Tn5-GDYN	This work
GR64-4	GR64 cured of pSfr64a and pSfr64b, Rif^R	This work
GR64-5	GR64-4/pRet42a::Tn5-GDYN	This work
GR64-6	GR64-4/pSfr64a::Tn5-GDYN	This work
CFN2001-1	CFN2001/pSfr64b::Tn5mob	This work
CFN2001-2	CFN2001/pSfr64b::Tn5mob, pRet42a::Tn5-GDYN	This work
CFN2001-3	CFN2001/pSfr64b::Tn5mob, pSfr64a::Tn5-GDYN	This work
*Escherichia coli*
DH5a	Receptor for transformation	[59]
S17-1	C600::RP-4-2 (Tc::Mu)(Km::Tn7)	[60]
S17/pDR21	Source of Tn5-GDYN	[17]
*Agrobacterium tumefaciens*
GMI9023	C-58 cured of its native plasmids	[35]
UIA143	recA pTi^- derivative of C58	[61]
GMI9023/pSfr64a	GMI9023 with pSfr64a::Tn5-GDYN	This work
GMI9023/pSfr64b	GMI9023 with pSfr64b::Tn5mob	This work
Plasmids
pSUP5011	Tn5mob	[60]
pRK2013	Conjugation helper	[36]

ISSN: 1471-2180