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Table 3 Reactivity of MAbs with different types of treated and untreated antigens as assessed by ELISA.

From: Characterization of surface proteins of Cronobacter muytjensiiusing monoclonal antibodies and MALDI-TOF Mass spectrometry

Type of antigen ** Treatment Absorbance (405 nm) ± SD *
   A1 B5 2C2 C5
OMP None 1.375 ± 0.20 0.720 ± 0.15 1.234 ± 0.58 1.481 ± 0.12
OMP 1% SDS + Boiling for 5 min 0.958 ± 0.07 0.492 ± 0.04 0.562 ± 0.08 0.901 ± 0.08
WC None 1.365 ± 0.08 0.565 ± 0.07 0.725 ± 0.08 0.835 ± 0.03
WC Heat 1.156 ± 0.16 0.423 ± 0.08 0.782 ± 0.03 1.026 ± 0.19
LPS None 0.553 ± 0.08 0.454 ± 0.04 0.425 ± 0.09 0.531 ± 0.04
None None 0.477 ± 0.05 0.469 ± 0.24 0.520 ± 0.07 0.412 ± 0.17
  1. OMP: outer membrane protein; WC: whole cell; LPS: Lipopolysaccharides, SD: Standard deviation.
  2. * Absorbance represents the average of two readings
  3. ** All antigens were prepared from C. muytjensii ATCC 51329