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Table 1 Bacterial strains and plasmids used in the present study

From: Role of the SaeRS two-component regulatory system in Staphylococcus epidermidisautolysis and biofilm formation

Strain or plasmid Relevant genotype or characteristic Reference or source
Strains   
E. coli DH5α λ- ϕ80dlacΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rK- mK-) supE44 thi-1 gyrA relA1 [49]
SE1457 Biofilm positive strain [50]
S. aureus RN4220 Restriction-negative, modification-positive isolate [51]
SE1457ΔsaeRS saeRS deletion mutant of strain 1457, Spcr This study
SE1457saec 1457ΔsaeRS complemented with saeRS This study
Plasmids   
pET-28a(+) Expression vector, KanR Novagen
pBT2
pCX19
Temperature-sensitive E. coli- Staphylococcus shuttle vector. Apr (E. coli) Cmr (Staphylococcus)
Derivate of pCX15
[52]
[53]
pMAD Escherichia coli/Staphylococcus Shuttle vector [54]
pMAD-saeRS Vector for allelic gene replacement of saeRS in S. epidermidis This study
pBT2-saeRS Vector for complementation of saeRS in S. epidermidis 1457ΔsaeRS This study
  1. *Abbreviations: Amp, ampicillin; Cm, chloramphenicol; Em, erythromycin.