BCM induces apoptosis and cell detachment in HKs. (A) Percentage of HKs staining positive for TUNEL. BCM induces significant levels of apoptosis in HKs after 4 and 24 hours of exposure while PCM does not. TUNEL data represents positive TUNEL cell counts over total cell counts. (B) Total cell counts obtained from propidium iodide stained HKs. After 24 hours of exposure to BCM, roughly half of the BCM treated HKs were still adhering to the culture well. Results represented as mean ± SD, n = 4, ** p < 0.01.