Table 10 Primers and probes for real-time PCR detection of virulence genes developed for this study
Target genea | Forward primer, reverse primer and probe sequences (5'-3') | Location within sequences | Gene Bank accession no. |
|---|---|---|---|
nleG6-2 (Z2150) | ATATGCTCTCTATATGATAAGGATG | 1928877-1928901 | AE005174 |
| Â | AAAGTGACATTCGTCTTTTCTCATA | 1928996-1928872 | Â |
| Â | [6FAM]CGTTAGTGCAACTTGTTGAAACTGGTGGAA[BHQ1] | 1928902-1928931 | Â |
nleG5-2 (Z2151) | AGACTATTCGTGGAGAAGCTCAAG | 1929199-1929222 | AE005174 |
| Â | TATTGAAGGCCAATCTGGATG | 1929337-1929317 | Â |
| Â | [6FAM]TGGATATTTTATGGGAAGTCTTAACCAGGATGG[BHQ1] | 1929269-1929301 | Â |
espK | ATTGTAACTGATGTTATTTCGTTTGG | 1673295-1673320 | AE005174 |
| Â | GRCATCAAAAGCGAAATCACACC | 1673419-1673397 | Â |
| Â | [6FAM]CAGATACTCAATATCACAATCTTTGATATATAAACGACC[BHQ1] | 1673330-1673368 | Â |
bfpA | CCAGTCTGCGTCTGATTCCA | 2756-2775 | FM180569 |
| Â | CGTTGCGCTCATTACTTCTGAA | 2816-2795 | Â |
| Â | TAAGTCGCAGAATGC-MGB | 2777-2791 | Â |