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Figure 4 | BMC Microbiology

Figure 4

From: High-resolution time series of Pseudomonas aeruginosa gene expression and rhamnolipid secretion through growth curve synchronization

Figure 4

Average growth curves, GFP expression and rhamnose secretion in strains NEG, QSN and IND. A) Average growth of NEG cells (black) with standard deviation (gray), inoculated at 0.0025 OD600 over two independent experiments. Average GFP expression, under the control of the PA01 rhlAB-promoter (green) with the standard deviation (light green) constructed from the same experiments. B) Average growth of QSN cells in the presence of 5 μM C4-HSL (black) with standard deviation (gray), inoculated at 0.0025 OD600 over two independent experiments. Average GFP expression, under the control of the PA01 rhlAB-promoter (green) with the standard deviation (light green) constructed from the same experiments. C) Average growth curve of IND cells (black) with standard deviation (gray), inoculated at 0.0025 OD600 over two independent experiments. NEG is not a reporter fusion strain, so there is no GFP expression. D) No rhamnose is detectable in NEG in two independent experiments (black and gray). E) Rhamnose is undetectable in QSN in the absence of C4-HSL in two independent experiments (black and gray squares), but is reconstituted in the presence of C4-HSL (black and gray triangles). F) Rhamnose secretion in IND in two independent experiments (black and gray squares). The inset shows the complete range of rhamnose secretion in IND cells under our experimental settings.

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