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Table 3 Identification and FISH reactivity profiles of five isolates from in sit u biofilms 013, 051 and 059

From: Phylogenetic group- and species-specific oligonucleotide probes for single-cell detection of lactic acid bacteria in oral biofilms

  Isolated strain (biofilm of origin)
  OMZ 1117 (013) OMZ 1118 (013) OMZ 1119 (051) OMZ 1120 (051) OMZ 1121 (059)
16S rRNA probes      
LGC358a 2-4 + 3-4 + 3-4 + 3-4 + 3 +
LAB759 + LAB759-comp - to 2 + 3-4 + 3-4 + 3 + - to 2 +
Lpla759 - - - - -
Lpla990+ H1018 - - - - -
L-Lbre466 - - - - -
L-Lbuc438 - - a - a - a -
Lcas467 - 4 + - 3-4 + -
Lsal574 - - - - -
L-Lsal1113 - - - - -
Lreu986 + H967 2-4 + - 3-4 + - 3-4 +
Lfer466 + H448 + H484 2-4 + - - - 3-4 +
L-Lcol732 - - - - -
Lvag222 - - 3-4 + - -
Lgas458 - - - - -
Lgas183 - - - - -
Identification c L. fermentum L. paracasei L.casei L. vaginalis L. paracasei L.casei L. fermentum
  1. a Positive at ≤ 45% formamide.
  2. b Scoring of fluorescence intensity is described in a footnote to Table 2.
  3. c Species identification was based on ≥ 99.5% 16S rRNA gene sequence similarity with reference strains listed in RDP10 (http://rdp.cme.msu.edu/index.jsp). The respective partial 16S rRNA gene sequences of OMZ 1117 and 1121 [EMBL: FR667951], and of OMZ 1118 and 1120 [EMBL: FR667952] were identical. OMZ 1119 was identified as L. vaginalis [EMBL: FR667953].