Skip to main content

Table 2 Reactivity of FISH probes to lactobacilli with target and non-target strains

From: Phylogenetic group- and species-specific oligonucleotide probes for single-cell detection of lactic acid bacteria in oral biofilms

   16S rRNA probes
Group, Strain OMZ LGC358a LAB759 + LABB759-comp Lpla759 Lpla990 + H1018 L-Lbre466-2 L-Lbuc438-2 Lcas467 Lsal574 L-Lsal1113-2 Lreu986 + H1018 Lfer466 + H448+ H484 L-Lcol732-2 Lvag222 Lgas458 Lgas183
L. buchneri et rel.                 
   L. plantarum FAM 1638 945 2-4+*,a 3-4+ 3-4+ 2-4+* - - - - - - - - - - -
   L. brevis ATCC 14869 625 3-4 + 2-3 + - - 4+ - - - - - ± -b - - -
   L. brevis OMZ 1114 1114 2-4+ 2-3+* - - 3-4+ - - - - - - -b - - -
   L. buchneri ATCC 4005 626 2-4 + 1-2 + - - - 3-4 + - - - - - -b - - -
   L. buchneri 1097 2-4 +* 2-3 +* - - - 3+ - - - - - -b - - -
L. casei et rel.                 
   L. casei ATCC 393 939 2-4+ 3-4+ - - - -c 3+ - - - - - - - -
   L. casei Cl-16 638 3-4 + 3-4 + - - - -c 3-4 + - - - - - - - -
   L. paracasei ATCC 25598 624 2-4 +* 2-4 +* - - - -c 3-4 +* - - - - - - - -
   L. rhamnosus AC 413 629 2-4 + 2-4 + - - - - 3-4 + - - - - - - - -
   L. rhamnosus ATCC 7469T 602 2-4 + 2-4 + - - - - 3 + - - - - - - - -
L. salivarius                 
   L. salivarius ATCC 11741 525 3-4+ 3-4+ - - - - - 2-4+ 3-4+ ± - - - - -
   L. salivarius OMZ 1115 1115 2-4+ - - - - - - 3-4+ 3-4+ - - - - - -
L. reuteri et rel.                 
   L. coleohominis DSM14060T 1113 1-3 + 2-4 + - - -d - - - - 3 + - 3-4 + - - -
   L. fermentum ATCC 14931 524 2-4 +* 2 +*, e - - - - - - - 2-4 + 3-4 + - - - -
   L. fermentum OMZ 1116 1116 2-4 + 2 +*, e - - - - - - - 2-4 + 3-4 + - - - -
   L. reuteri CCUG 33624T 1100 2-4 + 3-4 + - - - -c ± - - 2-4 + 2-4 + - - - -
   L. vaginalis UMCG 5837 1095 2-4 + 3-4 + - - - -c - - - 1-3 +* - - 3-4 + - -
L. gasseri et rel.                 
   L. acidophilus ATCC 4357 523 2-4+ 3-4+ - - - - - - - ± ± - - 2-4+ -
   L. crispatus ATCC 33820 522 3-4 + 3-4 + - - - - - - - -   - - 3-4 + -
   L. gasseri ATCC 19992 520 2-4 + 2-4 + - - - - - - - ± 1 + - - 1-3 + 2-4 +
   L. gasseri OMZ 549 549 2-4 + 2-4 + - - - - - - - ± - - - 2-4 + 2-4 +
   L. jensenii UMCG 20557 1096 2-4 + 3-4 + - - - - - - - - - - - - -
  1. * The asterisk indicates that only a small percentage of the cells could be stained by the probe, in spite of enzymatic pretreatment to improve probe penetration.
  2. a Fluorescence intensity was graded using an arbitrary five-step scale, where - (no fluorescence above background) and 1+ (very faint fluorescence) were considered negative signals, and 2+ (weak), 3+ (strong) and 4+ (brilliant fluorescence) were considered positive signals.
  3. b Probe L-Lcol732-2 labeled L. brevis and L. buchneri strains at formamide concentrations below 40%.
  4. c L-Lbuc438-2 cross-reacted with certain strains from the L. casei and L. reuteri groups at formamide concentrations of ≤ 45%.
  5. d L-Lbre466-2 was positive with L. coleohominis at ≤ 45% formamide in the hybridization buffer.
  6. e L. fermentum was stained with low intensity due to a weak mismatch at position 760.