Skip to main content

Table 2 Reactivity of FISH probes to lactobacilli with target and non-target strains

From: Phylogenetic group- and species-specific oligonucleotide probes for single-cell detection of lactic acid bacteria in oral biofilms

  

16S rRNA probes

Group, Strain

OMZ

LGC358a

LAB759 + LABB759-comp

Lpla759

Lpla990 + H1018

L-Lbre466-2

L-Lbuc438-2

Lcas467

Lsal574

L-Lsal1113-2

Lreu986 + H1018

Lfer466 + H448+ H484

L-Lcol732-2

Lvag222

Lgas458

Lgas183

L. buchneri et rel.

                

   L. plantarum FAM 1638

945

2-4+*,a

3-4+

3-4+

2-4+*

-

-

-

-

-

-

-

-

-

-

-

   L. brevis ATCC 14869

625

3-4 +

2-3 +

-

-

4+

-

-

-

-

-

±

-b

-

-

-

   L. brevis OMZ 1114

1114

2-4+

2-3+*

-

-

3-4+

-

-

-

-

-

-

-b

-

-

-

   L. buchneri ATCC 4005

626

2-4 +

1-2 +

-

-

-

3-4 +

-

-

-

-

-

-b

-

-

-

   L. buchneri

1097

2-4 +*

2-3 +*

-

-

-

3+

-

-

-

-

-

-b

-

-

-

L. casei et rel.

                

   L. casei ATCC 393

939

2-4+

3-4+

-

-

-

-c

3+

-

-

-

-

-

-

-

-

   L. casei Cl-16

638

3-4 +

3-4 +

-

-

-

-c

3-4 +

-

-

-

-

-

-

-

-

   L. paracasei ATCC 25598

624

2-4 +*

2-4 +*

-

-

-

-c

3-4 +*

-

-

-

-

-

-

-

-

   L. rhamnosus AC 413

629

2-4 +

2-4 +

-

-

-

-

3-4 +

-

-

-

-

-

-

-

-

   L. rhamnosus ATCC 7469T

602

2-4 +

2-4 +

-

-

-

-

3 +

-

-

-

-

-

-

-

-

L. salivarius

                

   L. salivarius ATCC 11741

525

3-4+

3-4+

-

-

-

-

-

2-4+

3-4+

±

-

-

-

-

-

   L. salivarius OMZ 1115

1115

2-4+

-

-

-

-

-

-

3-4+

3-4+

-

-

-

-

-

-

L. reuteri et rel.

                

   L. coleohominis DSM14060T

1113

1-3 +

2-4 +

-

-

-d

-

-

-

-

3 +

-

3-4 +

-

-

-

   L. fermentum ATCC 14931

524

2-4 +*

2 +*, e

-

-

-

-

-

-

-

2-4 +

3-4 +

-

-

-

-

   L. fermentum OMZ 1116

1116

2-4 +

2 +*, e

-

-

-

-

-

-

-

2-4 +

3-4 +

-

-

-

-

   L. reuteri CCUG 33624T

1100

2-4 +

3-4 +

-

-

-

-c

±

-

-

2-4 +

2-4 +

-

-

-

-

   L. vaginalis UMCG 5837

1095

2-4 +

3-4 +

-

-

-

-c

-

-

-

1-3 +*

-

-

3-4 +

-

-

L. gasseri et rel.

                

   L. acidophilus ATCC 4357

523

2-4+

3-4+

-

-

-

-

-

-

-

±

±

-

-

2-4+

-

   L. crispatus ATCC 33820

522

3-4 +

3-4 +

-

-

-

-

-

-

-

-

 

-

-

3-4 +

-

   L. gasseri ATCC 19992

520

2-4 +

2-4 +

-

-

-

-

-

-

-

±

1 +

-

-

1-3 +

2-4 +

   L. gasseri OMZ 549

549

2-4 +

2-4 +

-

-

-

-

-

-

-

±

-

-

-

2-4 +

2-4 +

   L. jensenii UMCG 20557

1096

2-4 +

3-4 +

-

-

-

-

-

-

-

-

-

-

-

-

-

  1. * The asterisk indicates that only a small percentage of the cells could be stained by the probe, in spite of enzymatic pretreatment to improve probe penetration.
  2. a Fluorescence intensity was graded using an arbitrary five-step scale, where - (no fluorescence above background) and 1+ (very faint fluorescence) were considered negative signals, and 2+ (weak), 3+ (strong) and 4+ (brilliant fluorescence) were considered positive signals.
  3. b Probe L-Lcol732-2 labeled L. brevis and L. buchneri strains at formamide concentrations below 40%.
  4. c L-Lbuc438-2 cross-reacted with certain strains from the L. casei and L. reuteri groups at formamide concentrations of ≤ 45%.
  5. d L-Lbre466-2 was positive with L. coleohominis at ≤ 45% formamide in the hybridization buffer.
  6. e L. fermentum was stained with low intensity due to a weak mismatch at position 760.