Phylogeography of Francisella tularensis subspecies holarctica from the country of Georgia

BMC Microbiology

Table 1 Melt-MAMA primers targeting informative canSNPs

SNP	SCHU S4 position	Genome SNP state (D/A)^a	Melt MAMA primer^c	Melt-MAMA primer sequences^d	Primer conc. (μM)	Annealing temp. (°C)	Melting T_m (°C)
B.Br.026	1484645	A/C	D	GAAACTTATTTGTTCCTAAGACAGTGACAcTA	0.800	55	73.1
			A	ggggcggggcggggcAAACTTATTTGTTCCTAAGACAGTGACAgTC	0.200		79.7
			C	GCATTGAGTTTGACAGGGTTGC	0.200
B.Br.027	1329722	T/G^b	D	ggggcggggcggggcggggcCATGCCAGGCACTACAATTGATAGTaTA	0.200	55	78.2
			A	TGCCAGGCACTACAATTGATAGTtTC	1.000		73.6
			C	TATACTTCTGACCATGGCGTTCAAAT	0.200
B.Br.028	212729	T/G	D	ggggcggggcggggcggggcAAATTAGTTCAAATGTTAAATTTGATcCT	0.200	55	75.8
			A	AAATTAGTTCAAATGTTAAATTTGATaCG	0.200		67.7
			C	CAAAATAAATCCCGTTGAGAATAGAA	0.200
B.Br.029	1185519	A/G	D	ggggcggggcggggcggggcTGCTTAATCTCATTGACTAGCTGTGgTA	0.200	55	78
			A	TGCTTAATCTCATTGACTAGCTGTGaTG	1.000		70
			C	ACAAAGTTGAAACTATCGAGCATAAATC	0.200
B.Br.030	928335	T/G	D	ggggcggggcggggcggggcTGTTGGGTCAAAGAGAGAAGTgTT	0.200	55	78.2
			A	ATTGTTGGGTCAAAGAGAGAAGTaTG	0.200		70
			C	GCCACCAAAGAATACAGAGTAGTCAT	0.200
B.Br.031	1634565	A/G	D	ggggcggggcggggcggggcGCACCAATCGTATCTAATTGATcCA	0.400	55	79
			A	GCACCAATCGTATCTAATTGATtCG	0.200		70
			C	AACTTTGCTAAAACAAATGCTGTTGC	0.200
B.Br.032	283540	A/G^b	D	ggggcggggcggggcggggcTGCTAAACCTACAGTAATCAGAAGTATtAT	0.200	55	72
			A	TGCTAAACCTACAGTAATCAGAAGTATcAC	0.600		68.4
			C	GCTAAATTTTAGTAAGATAAAAAGTGTAAGTAGTG	0.200

^aSNP states are presented according to their orientation in the SCHU S4 reference genome (NC_006570);
^bAssays designed from the reverse complement of the reference sequence.
^cD: Derived; A: Ancestral; C: Common Primer
^dPrimer tails and antepenultimate mismatch bases are in lower case

ISSN: 1471-2180