Table 1 Primers and probes used in this study.
Name (labelling) | Sequence (5' to 3') | Positions | Specificity | References |
|---|---|---|---|---|
PCR primers | ||||
Arch-21f | TTC CGG TTG ATC CYG CCG GA | 21-40 | Archaea | [28] |
Arch-958r | YCC GGC GTT GAM TCC AAT T | 958-976 | Archaea | [28] |
27f | AGA GTT TGA TCC TGG CTC AG | 27-46 | Eubacteria | [29] |
1492r | GGT TAC CTT GTT ACG ACT T | 1492-1510 | Eubacteria | [30] |
CARD-FISH probes | ||||
ANME1-350 | AGT TTT CGC GCC TGA TGC | 350-367 | ANME-1 archaea | [4] |
EelMS932 | AGC TCC ACC CGT TGT AGT | 932-949 | ANME-2 archaea | [4] |
ANME3-1249 | TCG GAG TAG GGA CCC ATT | 1250-1267 | ANME-3 archaea | [31] |
ANME3-1249H3 | GTC CCA ATC ATT GTA GCC GGC | 1229-1249 | Helper probe for ANME3-1249 | [32] |
ANME3-1249H5 | TTA TGA GAT TAC CAT CTC CTT | 1268-1288 | Helper probe for ANME3-1249 | [32] |
DSS658 | TCC ACT TCC CTC TCC CAT | 658-685 | Desulfosarcina spp., Desulfofaba spp., Desulfococcus spp., Desulfofrigus spp. | [33] |