|
PCR primers
|
|
Arch-21f
|
TTC CGG TTG ATC CYG CCG GA
|
21-40
|
Archaea
|
[28]
|
|
Arch-958r
|
YCC GGC GTT GAM TCC AAT T
|
958-976
|
Archaea
|
[28]
|
|
27f
|
AGA GTT TGA TCC TGG CTC AG
|
27-46
|
Eubacteria
|
[29]
|
|
1492r
|
GGT TAC CTT GTT ACG ACT T
|
1492-1510
|
Eubacteria
|
[30]
|
|
CARD-FISH probes
|
|
ANME1-350
|
AGT TTT CGC GCC TGA TGC
|
350-367
|
ANME-1 archaea
|
[4]
|
|
EelMS932
|
AGC TCC ACC CGT TGT AGT
|
932-949
|
ANME-2 archaea
|
[4]
|
|
ANME3-1249
|
TCG GAG TAG GGA CCC ATT
|
1250-1267
|
ANME-3 archaea
|
[31]
|
|
ANME3-1249H3
|
GTC CCA ATC ATT GTA GCC GGC
|
1229-1249
|
Helper probe for ANME3-1249
|
[32]
|
|
ANME3-1249H5
|
TTA TGA GAT TAC CAT CTC CTT
|
1268-1288
|
Helper probe for ANME3-1249
|
[32]
|
|
DSS658
|
TCC ACT TCC CTC TCC CAT
|
658-685
|
Desulfosarcina spp., Desulfofaba spp., Desulfococcus spp., Desulfofrigus spp.
|
[33]
|
