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Table 3 IS629 element presence/absence in CC strains from the O157:H7 stepwise evolutionary model

From: Prevalence, distribution and evolutionary significance of the IS629 insertion element in the stepwise emergence of Escherichia coliO157:H7

      A6         A5      A4     A2   A1   A?   A6
NR Phage Or backbone 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27
IS.1 Sp 4 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND
IS.2 Sp 4 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND
IS. 3 Sp 5 stx2 + - - - - - - - - - - - - - - - - - - - - - - - - + -
IS. 4 SpLE 1 - + - - - - + - - - - - - - - - - - - - - - - - - - -
IS. 5 SpLE 1 + + + + + - + - - - - - - - - - - - - - - - - - - + +
IS. 6 SpLE 1 - + - - - - + - - - - - - - - - - - - - - - - - - - -
IS. 7 SpLE 1 + + + + + - + + - - - - - - - - - - - - - - - - - + +
IS. 8 Sp 8 + - + - + - + - - - - - - - - - - - - - - - - - - + -
IS. 9 Sp 8 - + - - - - - - - - - - - - - - - - - - - - - - - - +
IS. 10 back + + - - + - + + - - - - - - - - - - - - - - - - - + +
IS. 11 back + + - - + - - - - - - - - - - - - - - - - - - - - + +
IS. 12 Sp 12 + - - - - - - - - - - + + + - - - - - - - - - - - + -
IS. 13 back + + + + + + + - - - - - - - - - - - - - - - - - - + +
IS. 14 Sp 13 + + + + + + - + - - - - - - - - - - - - - - - - - + +
IS. 15 Sp 14 + + + + + + + + - - - - - - - - - - - - - - - - - + -
IS.16 SpLE 2 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND
IS. 17 back + + - - - - - - - - - - - - - - - - - - - - - - - + +
IS. 18 Sp 15 stx1 + + - - + - - - - - - - - - - - - - - - - - - - - + +
IS. 19 back + - + + + - - + - - - - - - - - - - - - - - - - - + -
IS. 20 Sp 17 + - + + + - + + - - - - - - - - - - - - - - - - - + -
IS. 21 SpLE3 + + - + + + + + - - - - - - - - - - - - - - - - - + +
IS.22 back ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND
IS. 23 SpLE 5 + + - - + - + + - - - - - - - - - - - - - - - - - + +
IS. 24 SpLE 1 - + - - - - - - - - - - - - - - - - - - - - - - - - +
IS. 25 SpLE 1 - + - - - - - - - - - - - - - - - - - - - - - - - - +
IS.26 933O ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND
IS. 27 SpLE 2 - + - - - - - - - - - - - - - - - - - - - - - - - - +
IS.28 933Y ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND
IS. 29 Sp 1 - - + + - - - + - - - - - - - - - - - - - - - - - - -
IS. 30 Sp 4 - - + - - - - - - - - - - - - - - - - - - - - - - - -
IS. 31 Phage - - + + - - - - + + - + + + - - - - - - - - - - - - -
IS. 32 back - - + + - - - + - - - - - - - - - - - - - - - - - - -
IS. 33 Sp 13 - - + + - - - - - - - - - - - - - - - - - - - - - - -
IS. 34 back - - + + - - - - - - - - - - - - - - - - - - - - - - -
IS. 35 Sp 5 stx 2 - - + + - - - + - - - - - - - - - - - - - - - - - - -
IS.36 back ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND
IS. 37 Phage - - + + - - - - - - - - - - - - - - - - - - - - - + -
IS. 38 back - - + + - - - - - - - - - - - - - - - - - - - - - - -
IS. 39 (gne gene) - - - - - + - - - - - - - - - - - - - - - - - - - - -
IS. 40 pO157 + - - - + - - - - - - - - - - - - - - - - - - - - + -
IS. 41 pO157 + + + + + + + + - - - - - - - - - - - - - - - - - + +
IS. 42 pO157 - - + + - - - - - - - - - - - - - - - - - - - - - + +
IS.43 pO157                            
IS. 44 pO157 - - + + - - - - - - - - - - - - - - - - - - - - - - -
IS. 45 pO157 - - - + - - - - - - - - - - - - - - - - - - - - - - -
IS. 46 back - - - + - - - - + + - - - - - - - - - - - - - - - - -
IS.47 back ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND
IS.48 pO157 ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND ND
  1. IS629 sites were numbered from 1 - 47 (NR) starting with all sites in Sakai, followed by all additional, unshared sites from EDL933, EC4115, the sites found in the plasmids and unshared sites of strain TW1435. The newly found IS629 insertion in O rough:H7 strain MA6 was numbered IS.39 [4]. A1 - A6 are strains belonging to the different clonal complexes.
  2. Sp - Phage; SpLE - Phage-like element; back - backbone; ND -Not determined, primers failed to amplify the region.