Figure 2

Hierarchical clustering of mixed samples demonstrates the resolution capabilities of the UBDA array. This dendogram and heat map illustrates a unique bio-signature pattern obtained from Lactobacillus plantarum, mixed sample (synthetic mixture in a 4:1 ratio of L. plantarum and Streptococcus mitis), S. mitis, mixed sample (a synthetic mixture of L. plantarumand S. mitisgenomic DNA in a ratio of 4:1 with a spike-in of pBluescript plasmid at 50 ng) and pBluescript plasmid. Normalized data from the 9-mer data set were filtered for intensity signals greater than the 20^th percentile. Only intensity signals with a fold change of 5 or greater were included. These 36,059 elements were subjected to hierarchical clustering with Euclidean distance being used as a similarity measure. The signal intensity values were represented on a log₂ scale and range from 8.4 to 13.4.