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Table 1 Differentially regulated proteins of X.dendrorhous in MM glucose.

From: Proteomic analysis of the carotenogenic yeast Xanthophyllomyces dendrorhous

  

cNormalized average spot quantity

dFold change

aSSP

bDescription

Lag

Exponential

Stationary

E/L

S/L

  

Avg.

SD

Avg.

SD

Avg.

SD

  
 

Cellular Processes:

Transport and motor proteins

        

6818

Putative coatomer subunit alpha

144

111

813

345

1195

155

5.64

8.30

8703

Myosin-associated protein

152

151

995

598

735

255

6.56

4.84

8711

 

623

441

3145

2255

2459

906

5.05

3.95

5719

Golgi transport protein

7637

435

2446

1101

7415

1660

-3.12

-1.03

5728

 

4330

676

1390

618

3494

1095

-3.12

-1.24

6703

 

9226

2086

4269

306

7877

3334

-2.16

-1.17

2712

SS1G_01912

13322

4086

3886

2574

5444

711

-3.43

-2.45

7403

KIP1 kinesin-related protein

1494

866

5246

2780

3349

528

3.51

2.24

7804

Vacuolar-sorting-associated protein 25

3952

977

11351

6299

3428

1137

3.57

5.03

 

Environmental Information Processing:

Signal Transduction

        

3814

Serine/threonine-prot. phosphatase PP1-1

472

451

270

108

2273

1825

-1.75

4.81

3815

 

14950

1985

7701

6806

10797

2018

-5.54

1.66

3816

 

208

94

133

103

745

415

-1.57

3.57

5724

Nucleotide phosphodiesterase

356

91

966

339

607

196

2.72

1.71

0126

14-3-3. DNA damage checkpoint protein

636

515

98

102

2338

2264

-6.49

3.68

0127

 

261

327

236

252

3161

937

-1.11

12.09

0128

 

85

79

253

101

904

339

2.98

10.64

 

Genetic Information Processing

        

9206

Ribosomal_L15

19280

5898

6131

5697

9959

8398

-3.14

-1.94

7815

Mediator of RNA polymerase II

1436

1029

2487

788

3794

542

1.73

2.64

6707

Hypothetical protein. DNA helicase

1663

234

785

319

2342

1310

-2.12

1.25

6610

Replication factor C subunit 3

1663

234

785

319

2342

1310

-2.12

1.41

3228

G4P04 (Fragment)

12049

2891

7896

4292

2188

1579

-1.53

-5.51

4803

Calpain-like protease palB/RIM13

1155

494

1308

890

347

171

1.13

-3.33

  

2072

391

2087

1350

1715

101

1.01

-1.21

7528

Serine/threonine protein kinase (Kin28)

1366

369

2405

840

3280

802

1.76

2.40

7515

Histone acetyltransferase, predicted

3162

819

10965

2273

9410

1514

3.47

2.98

7711

Cell division control protein 25, putative

957

73

2201

1398

2842

659

2.30

2.97

 

Metabolism

        

7407

UDP-xylose synthase

5850

468

6499

2421

12649

295

1.11

2.16

8507

ATP synthase subunit alpha

13682

2423

11233

8105

4099

3058

-1.22

-3.34

7801

Heat shock protein, putative

1059

268

4202

2317

2373

708

3.97

2.24

 

Lipid and Carbohydrate Metabolism

        

2523

Acetyl-CoA carboxylase

10538

888

5524

2209

10218

5489

-1.91

-1.03

2524

 

26474

7704

15933

13733

17308

4885

-1.66

-1.53

3516

 

38053

5148

12837

8209

26762

5654

-2.96

-1.42

7519

Phosphoglucomutase-1

1967

565

6358

1401

2562

632

3.23

1.30

2319

Acetyl-CoA synthetase

14327

8064

11303

10213

4218

576

-1.27

-3.40

4104

ATP-citrate synthase

18720

2582

14847

10388

11099

2402

-1.26

-1.69

4413

ATP-citrate lyase

9657

987

6925

7702

8736

2536

-1.39

-1.11

6604

Fatty acid synthase

1291

149

285

315

1978

483

-4.52

1.53

 

Secondary Metabolite/

Carotenoid Biosynthesis

        

4515

Phytoene/squalene synthetase

5412

2656

13551

3057

7789

1051

2.50

1.44

4609

Mevalonate kinase

425

96

283

243

1246

454

-1.50

2.93

3517

Phosphomevalonate kinase

1005

494

270

220

367

504

-3.72

-2.73

6308

Diphosphomevalonate decarboxylase

2146

1521

4628

2509

5598

1347

2.16

2.61

 

Redox Metabolism

        

4401

Hypothetical oxidoreductase

6305

1432

1034

1014

1432

561

-6.10

-4.40

3606

Putative protein Cu-oxidase

741

92

184

195

1198

691

-4.04

1.62

5202

SDR family

2593

668

342

91

3515

418

-7.59

1.36

5208

Alcohol dehydrogenase

2564

1239

1008

1032

1607

578

-2.54

-1.60

4713

Monooxygenase

3930

522

4267

1706

5044

500

1.09

1.28

5703

 

4713

612

6594

2637

8287

916

1.40

1.76

5315

Cytochrome P450

10876

4259

16346

15386

6649

4692

1.50

-1.64

7108

Mn SOD

12020

3850

18262

13048

11032

1547

1.52

-1.09

 

Amino Acid Metabolism

        

8604

Seryl-tRNA synthetase

783

87

2517

1567

3861

203

3.21

4.93

7209

Methionyl-tRNA formyltransferase

912

290

28686

4392

17584

6195

31.44

19.27

7210

 

4348

1880

15379

2474

9085

2322

3.54

2.09

7816

Kynurenine 3-monooxygenase

111

73

726

424

811

64

6.56

7.33

7817

 

114

119

1139

751

1367

206

10.02

12.03

7819

 

130

84

1625

1134

1797

821

12.50

13.82

6821

Aspartyl-tRNA synthetase

156

81

395

76

1532

796

2.54

9.84

6828

 

580

11

2001

1020

2199

706

3.45

3.79

5410

Probable acetylornithine aminotransferase

4766

986

1794

1531

2615

447

-2.66

-1.82

2517

Phenylalanyl-tRNA synthetase beta chain

3325

375

813

639

2104

1397

-4.09

-1.58

5409

Glutamate dehydrogenase

2194

1506

2738

930

6893

2363

1.25

3.14

 

Unknown

        

2709

Conserved hypothetical protein

5609

2745

1227

889

4692

657

-4.57

-1.20

2710

 

2584

1482

1157

1630

1465

1413

-2.23

-1.76

6603

Hypothetical protein

3640

575

1014

1091

2985

120

-3.59

-1.22

7306

Hypothetical protein

2652

601

795

253

3569

2539

-3.34

1.35

6110

YALI0D17292p

10346

2105

1204

1434

8343

763

-8.59

-1.24

3503

Predicted protein

2670

367

906

897

735

650

-2.95

-3.63

  1. a SSP numbers were assigned by PDQuest software analysis.
  2. b Identifications were obtained using the Swiss-Prot and KEGG Pathways databases and contigs of X. dendrorhous genomic DNA.
  3. c Data derived from PDQuest estimation.
  4. d Mean fold changes compared with the 24 h cultures. Bold values indicate p < 0.01, italic p < 0.02 and underlined values indicate p < 0.05. Avg., average; SD, standard deviation.