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Figure 2 | BMC Microbiology

Figure 2

From: Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexaconfers enhanced adhesion to cultured cells and fibronectin

Figure 2

Surface localization of LigA and LigB. Surface immunofluorescence assay was performed with L. biflexa wild-type strain (Patoc wt), and ligA- (Patoc ligA), and ligB- (Patoc ligB) L. biflexa transformants. Strains were labeled with normal rabbit serum (control) and antibodies against LigA (LigANI), LigB (LigBNI), whole leptospires, and GroEL. A DAPI counterstain was used to document the presence of leptospires. A photomicrograph is shown from one of three representative experiments.

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