LigA and LigB expression in L. biflexa. A. Schematic diagram of plasmid constructs used to express constitutively LigA and LigB. The determinants for replication in L. biflexa (parAB and rep), as well as a spectinomycin (SpcR)- resistance cassette is indicated. B. Western blot of whole-cell lysates of L. interrogans serovar Copenhageni strain Fiocruz L1-130 (Fiocruz wt), L. biflexa serovar Patoc strain Patoc 1 (Patoc wt), and L. biflexa serovar Patoc strain Patoc 1 electrotransformed with pSLEPFligA (Patoc ligA) and pSLEPFligB (Patoc ligB) obtained by using LigA/B antiserum.
The positions of standard molecular mass markers (in kilodaltons) are indicated on the left.