Expression of P
during YgjD depletion. Single cell measurements of cell elongation rate and GFP fluorescence of two strains with transcriptional reporters for Papt (A and B) and Prsd (C and D). Each point represents a measurement for a single cell. In both strains, cell elongation rate decreased with increasing generations during YgjD depletion as shown in Figures 1B and 2A. A) and B) Papt is repressed by (p)ppGpp; its expression decreases during YgjD depletion, and decreases steadily with decreasing cell elongation rate. C) and D) Prsd is induced by (p)ppGpp; its expression increases during YgjD depletion, and steadily increases with decreasing cell elongation rate.