Polypeptide tested
|
ELISA* cell-free supernatant
|
ELISA* purified 6xHis polypeptide
|
SPR† purified 6xHis polypeptide
|
---|
ΔNarG
|
-
|
-
|
-
|
ΔFnBPA
|
Fn
|
-
|
Fn
|
ΔEbh
|
Fn
|
Fn
|
(Fn), Fg
|
ΔCoa
|
(Fn), Fg
|
(Fn), Fg,
|
Fg
|
ΔPurK
|
Fn, Fg
|
Fn, (CI)
|
Fn, Fg
|
ΔSCOR
|
(Fn), (Fg)
|
ND
|
ND
|
ΔUsp
|
Fn, Fg, (CIV)
|
(Fn)
|
Fg
|
ΔIspD
|
(Fn), (Fg)
|
ND
|
ND
|
ΔPBP
|
(Fn), (Fg)
|
-
|
-
|
D1-D3‡
|
Fn
|
ND
|
ND
|
- * Binding of polypeptides to immobilized target molecules by enzyme-linked immunoassay
- † Binding of polypeptides to immobilized target molecules by surface plasmon resonance
- ‡ C-terminally FLAG-tagged recombinant polypeptide used as positive control in screening of the FTP library
- - indicate no binding; ligand in brackets indicate weak binding
- ND, indicate not determined. The SCOR and IspD polypeptides could not be produced as 6xHis recombinant polypeptides and the D1-D3 polypeptide was produced into the cell-free growth medium and did not carry a His tag.