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Table 2 SPR analysis of His6-polypeptides

From: Adhesive polypeptides of Staphylococcus aureus identified using a novel secretion library technique in Escherichia coli

Polypeptide

KD to Fn (M) *

KD to Fg (M) *

His-ΔNarG

0,77

0,72

His-ΔFnBPA

5,24 × 10 -6

0,31

His-ΔEbh

0,02

1,25 × 10 -6

His-ΔCoa

< 0†

1,80 × 10 -7

His-ΔPurK

4,43 × 10 -7

5,39 × 10 -6

His-ΔUsp

0,35

6,45 × 10 -6

His-ΔPBP

0,36

0,13

  1. * the steady state affinity constants (KD) of the seven analytes tested are shown in molar concentrations; values shown in bold indicate high affinity for the indicated ligand (Fn or Fg).
  2. † affinity was not measurable since all values were negative