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Table 2 SPR analysis of His6-polypeptides

From: Adhesive polypeptides of Staphylococcus aureus identified using a novel secretion library technique in Escherichia coli

Polypeptide KD to Fn (M) * KD to Fg (M) *
His-ΔNarG 0,77 0,72
His-ΔFnBPA 5,24 × 10 -6 0,31
His-ΔEbh 0,02 1,25 × 10 -6
His-ΔCoa < 0† 1,80 × 10 -7
His-ΔPurK 4,43 × 10 -7 5,39 × 10 -6
His-ΔUsp 0,35 6,45 × 10 -6
His-ΔPBP 0,36 0,13
  1. * the steady state affinity constants (KD) of the seven analytes tested are shown in molar concentrations; values shown in bold indicate high affinity for the indicated ligand (Fn or Fg).
  2. † affinity was not measurable since all values were negative
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BMC Microbiology

ISSN: 1471-2180

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