|
Intra-assay 1
|
Inter-assay 2
|
---|
|
C. coli
|
C. jejuni
|
C. coli
|
C. jejuni
|
---|
Genome copy number
|
CVc(%)
|
Ct range
|
CVj(%)
|
Ct range
|
CVc(%)
|
Ct range
|
CVj(%)
|
Ct range
|
---|
10
8
|
2.27
|
14.18-15.25
|
5.63
|
14.18-17.15
|
4.89
|
13.86-16.11
|
1.94
|
14.30-15.01
|
10
7
|
1.33
|
16.63-17.71
|
0.95
|
17.55-18.21
|
4.69
|
16.33-17.88
|
0.83
|
17.86-18.27
|
10
6
|
1.99
|
20.05-20.78
|
1.13
|
21.02-21.81
|
3.42
|
19.29-21.80
|
1.37
|
21.15-22.04
|
10
5
|
1.60
|
23.32-24.63
|
0.57
|
24.15-24.69
|
4.08
|
23.22-25.55
|
0.67
|
24.01-24.48
|
10
4
|
0.81
|
26.92-28.07
|
0.35
|
26.56-26.91
|
1.52
|
26.23-28.48
|
2.65
|
26.64-28.30
|
10
3
|
1.28
|
30.44-31.28
|
0.53
|
30.11-30.69
|
1.90
|
29.70-31.37
|
1.99
|
28.60-30.85
|
10
2
|
1.22
|
33-37-34.82
|
0.40
|
33.66-34.05
|
2.46
|
33.80-35.78
|
1.39
|
33.62-34.60
|
10
1
|
0.87
|
37.29-38.66
|
2.21
|
35.65-37.77
|
3.10
|
37.10-38.91
|
2.21
|
36.11-37.43
|
CFU/g of faeces
|
CV
c
(%)
|
Ct range
|
CV
j
(%)
|
Ct range
|
CV
c
(%)
|
Ct range
|
CV
j
(%)
|
Ct range
|
2 × 10
8
|
3.23
|
17.22-18.35
|
2.28
|
18-74-19.81
|
-
|
-
|
-
|
-
|
2 × 10
7
|
1.33
|
20.60-21.15
|
2.53
|
20.57-22.02
|
0.75
|
19.54-19.88
|
1.21
|
21.65-22.27
|
2 × 10
6
|
1.89
|
24.08-24.97
|
0.91
|
24.13-24.62
|
2.37
|
23.51-24.85
|
0.70
|
24.15-24.60
|
2 × 10
5
|
1.15
|
27.23-28.38
|
1.40
|
27.02-28.45
|
0.57
|
26.40-26.79
|
1.46
|
27.04-28.69
|
2 × 10
4
|
2.20
|
28.28-29.75
|
1.98
|
30.13-31.80
|
2.58
|
28.00-29.90
|
2.10
|
30.7-32.31
|
2 × 10
3
|
4.40
|
32.20-33.77
|
1.62
|
34.61-35.96
|
2.07
|
32.00-33.22
|
1.80
|
34.48-36.45
|
2 × 10
2
|
4.38
|
34.61-37.78
|
1.76
|
38.04-39.37
|
1.64
|
35.35-36.56
|
1.92
|
37.34-39.03
|
- The coefficients of variation (CV) of the threshold cycles values (Ct) were evaluated for the C. coli real-time PCR (CVc) and for the C. jejuni real-time PCR (CVj). For each CVc and CVj, the range of Ct (Ct range), which corresponds to the smallest and the highest values of the Ct found among the ten, was indicated for each dilution for both intra-and inter-assay testings.
- 1 Results of intra-assay testing: ten replicates of each sample were tested in one PCR run
- 2 Results of inter-assay testing: one replicate of each sample was tested once in each of ten different PCR runs