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Figure 5 | BMC Microbiology

Figure 5

From: The MP65 gene is required for cell wall integrity, adherence to epithelial cells and biofilm formation in Candida albicans

Figure 5

Adhesion analysis of the mp65Δ mutant. (A) Adhesion of the mp65Δ mutant to BEC. Representative fields randomly selected showing the interaction between yeast cells [wild type (wt), mp65Δ mutant (hom) and revertant (rev) strains] and BEC after 1 h of incubation at 37°C. The magnification bar corresponds to 100 μm. See the Methods section for more details. (B) Adhesion assay data. Histograms showing the adherence of the wild type (wt: black column), mp65Δ mutant (hom: grey column) and revertant (rev: white column) strains to BEC. The bars indicate the standard errors. Significant differences from wild type adhesion (P < 0.05) are indicated by asterisks. (C) Adhesion of the mp65Δ mutant to Caco-2 cell monolayers. Recovery of Candida cells [wild type (wt: black column), mp65Δ mutant (hom: grey column) and revertant (rev: white column) strains] at different time points (30 and 60 min) of incubation with Caco-2 cells. Adherent cells recovered after thorough washing out of the microplate (Panel 1). Non-adherent cells recovered from the supernatant (Panel 2). The results are the mean of 3 independent experiments. The bars indicate the standard deviations.

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