Pair-wise comparisons of functional gene groups from swine versus other gut metagenomes. Pair-wise comparisons were calculated for the pig fecal metagenome versus (A) lean mouse cecum (B) cow rumen (C) human adult (D) termite gut (E) human infant (F) fish gut (G) and chicken cecal metagenomes is shown. Each point on this exploratory plot represents a different SEED Subsystem and it's relative abundance within the pig fecal metagenome compared to other available gut metagenomes within the MG-RAST database. Points closer to y-axis represent functions more abundant in the swine gut metagenome, while points closer to the x-axis are more abundant in other gut metagenomes. Points laying on or near the dotted midline have equal or very similar abundances within both metagenomes. A matrix of the abundance of sequences assigned to each SEED Subsystem from each gut metagenome was generated using the "Metabolic Analysis" tool in MG-RAST. The number of reads from each individual pig, human infant, and human adult metagenomes were each combined since there was more than one metagenome for each of these hosts within the MG-RAST database. The e-value cutoff for metagenomic sequence matches to SEED Subsystems was 1×10-5 with a minimum alignment length of 30 bp. Fisher exact tests were used with the Benjamin-Hochberg FDR multiple test correction to generate a list of significantly different SEED Subsystems using STAMP v1.0.2 software . The Newcombe-Wilson method was used to calculate the 95% confidence intervals.