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Table 4 Transposon mutants screened with the LPS specific phage JG004.

From: Sequencing and Characterization of Pseudomonas aeruginosa phage JG004

Transposon mutant

Integration of transposon

Product name*

Pathway and Literature

TM3

speD (PA0654)

S-adenosylmethionine decarboxylase proenzyme

Polyamine biosynthesis (PseudoCyc)

TM4

PA0534

conserved hypothetical protein

-

TM7

PA0421

hypothetical protein

-

TM12

wzz2 (PA0938)

hypothetical protein

Involved in O-antigen chain length determination/LPS biosynthesis [57]

TM13

PA2555

probable AMP-binding enzyme

-

TM15

algC (PA5322)

phosphomannomutase

LPS biosynthesis; alginate biosynthesis [16]

TM16

migA (PA0705)

alpha-1,6-rhamnosyl-transferase

LPS outer core biosynthesis [42]

TM17

rmlA (PA5163)

glucose-1-phosphate thymidylyltransferase

LPS biosynthesis [39]

TM18

waaL (PA4999)

O-antigen ligase

LPS biosynthesis [58]

TM19

PA5001

hypothetical protein

LPS biosynthesis [40]

TM20, TM22

rmlB (PA5161)

dTDP-D-glucose 4,6-dehydratase

LPS biosynthesis [40]

TM21

PA2200

conserved hypothetical protein

-

TM23

wapR (PA5000)

alpha-1,3-rhamnosyl-transferase WapR

LPS outer core biosynthesis [42]

  1. Integration of the transposon was identified by arbitrary PCR.* = according to http://www.pseudomonas.com