Sequencing and Characterization of Pseudomonas aeruginosa phage JG004

BMC Microbiology

Table 1 Strains and phages used in this study.

Bacterial strain or phage	Phenotype or genotype	Reference
PAO1*	Wild type	[54]
PA14	Wild type	[55]
PAO1 ΔmucA*	PAO1 mucA::aacC1-gfp Gm^R	Sabrina Thoma, this laboratory, unpublished
PAO1 ΔpilA*	pilA inactivated by allelic displacement; tagged with eGFP, Tc^R, Gm^R	[56]
PAO1 ΔfliM *	fliM inactivated by allelic displacement; tagged with eGFP, Tc^R, Gm^R	[56]
PAO1 ΔalgC	algC::aacC1-gfp Gm^R	Julia Garbe, this laboratory, unpublished
BT2, BT72, BT73, RN3, RN43, RN45*, NN84	Clinical CF isolates	Medical Highschool Hannover, Germany
PACF15, PACF21*, PAKL1,	Clinical CF isolates	Gerd Döring,
PAKL4, PACF60, PACF61, PACF62, PACF63		Tübingen, Germany
Nr. 18, 19, 26*, 29	Urinary tract infection isolate	Michael Hogardt, München, Germany
JG004	Wild type PAO1 LPS-specific lytic bacteriophage	This study

* = strains infected by phage JG004 in the host range and receptor studies.
Abbreviations: Gm^R, resistant to gentamicin; Tc^R, resistant to tetracyclin; eGFP, enhanced green fluorescent protein; LPS, lipopolysaccharide.

ISSN: 1471-2180