Phenotypic characterisation of hydrophobin mutants. A: Germination rates under different conditions, 24 h.p.i. I, II, II: Three transformants of hydrophobin triple mutant. Standard deviations are shown. B: Sclerotia formation on Gamborg agar plates. C: Germinated sclerotia with conidiophores and macroconidia (scale bar: 3 mm). D: Microconidia (hollow arrows) produced on phialides (filled arrows). Phialides were observed on branching hyphae and on macroconidia of B05.10 and the triple mutant (scale bar: 10 μm). E: Penetration into heat-killed onion epidermal cell layers (16 h.p.i). Fungal structures at the epidermal surface were stained with trypan blue (scale bar: 25 μm). F: Lesion formation on detached tomato leaves. Standard deviations are shown. G: Wettability test with water and 0.2% SDS on non-sporulating mycelia. Pictures were taken after 3 h. H: Wettability test with SDS solutions on sporulating aerial mycelia. Pictures were taken after 7 h.