Sequence alignments and hydropathy plots of B. cinerea hydrophobins and confirmed class I and II hydrophobins. A: Amino acid alignment of Bhp1 and class I hydrophobins. B: Amino acid alignment of Bhp2/3 and class II hydrophobins. The signal peptides are underlined. Hcf3 (Acc.: CAD92803) and Hcf5 (Acc.: CAC27408) from Cladosporium fulvum; Hyd5 (Acc.: AAN76355) from Fusarium verticillioides; Mpg1 (Acc.: P52751) and Mhp1 (Acc.: AAD18059) from M. oryzae; Xph1 (Acc.: CAC43386) from X. parietina. C and D: Hydropathy plots with Bhp1 and M. oryzae Mpg1 (left), and with Bhp2, Bhp3 and M. oryzae Mhp1 (right). Hydropathy values were calculated for the sequences covering the eight cysteines (window size for calculation: 7 amino acids). Positive values indicate regions of high hydrophobicity. Positions of cysteine residues are marked by triangles. Grand average of hydropathicity (GRAVY) of the analysed region is indicated in parentheses.